Rhodamine B, Spectrum [Rhodamine B] | AAT Bioquest, Study the spectroscopic characteristics of Rhodamine B Dye …
It is sometimes also known as Basic Violet 10. Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.
Green excitation, Yellow / Red emission Filters(Rhodamine B) Exciter is a BP545/25. Dichroic mirror is FT570. Emission (barrier) Filter is a BP605/70. Absorption and emission spectra is for Rhodamine B fluorochrome.
Keywords: fluorescence spectrum , Rhodamine B , laser dye, concentration effect, theoretical model 1. INTRODUCTION The optical excitation of a molecule and its subsequent emission of spontaneous luminescence or stimulated radiation results in a series of intermolecular processes, such as orientation or translational, which occur in both, Absorption/ Emission Spectra Of Rhodamine B In Ethanol. … Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for …
The resulting fluorescent derivative exhibits an excitation maximum at a wavelength of 556 nm and a maximal emission wavelength of 580 nm when dissolved in methanol. In the same solvent, the compound has an extinction coefficient of approximately 75,000 M ?1 cm ?1. Sign in to download full-size image. Figure 10.19.
Rhodamine B is an organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome. It has a role as a fluorochrome, a fluorescent probe and a histological dye.
